The study of trace metals and radionuclides has gained impetus due to their health hazardous potential in an environment. The physico-chemical, trace metal and radioactivity assessment in soils of western Himalaya (across Jammu, Kashmir and Ladakh) has been put forth in this study. The main objectives are to evaluate trace metal distribution and activity concentrations of radionuclides in soil for possible ecological deterioration and human health risk assessment in the region. The Co, Cr, Mn, Ni and Zn were analyzed using inductively coupled plasma atomic emission spectrometry, while Cd, Cu and Pb analyzed in Differential Pulse Anodic Stripping Voltammetry. For radionuclide activity concentration, samples were analyzed using 3″ × 3″ NaI (Tl) gamma ray system. The soil analyzed is characterized as strongly acidic to slightly alkaline with trace metal concentration in decreasing order of Fe, Mn, Zn, Ni, Cu, Cr, Co, Pb and Cd. Cu and Co concentration is found higher in agricultural/horticultural soils; however, Cd shows moderate to significant enrichment in all soils. Areas with volcanic lithology and extensively engaged in horticulture and agricultural activities exhibit higher ⁴⁰K activity concentration. Comparatively higher terrestrial and total gamma dose is observed in Sonamarg and Pangong areas, respectively. The radiation indices (AUI, RLI, Hex and Hin) affirm the suitability of material for utilization. Excess lifetime cancer risk (ELCR_(t)) from total dose (terrestrial + cosmic) exceeds world average in all the observed locations. The study details out the agricultural and horticultural soils as highly contaminated which may result in deleterious impact on eco-biological environment.