Voltammetry measurements have been employed to investigate the redox behaviour of curcumin in aqueous media using functionalized carbon nanotube (FCNTs) modified glassy carbon electrode (GCE). The electro-catalytic properties of FCNTs modified electrode are superior in comparison to the conventional electrode in generating the electrochemical response from curcumin. The oxidation process of the curcumin over the modified substrate is found to be pH dependent and shows 2e⁻ and 2H⁺ proton transfer electrochemical process. The oxidation peak is obtained at 0.37 V and the peak current is found to be linear with the varying concentration of curcumin. The limit of detection (LOD) and the limit of quantification (LOQ) for the curcumin are obtained as 60 and 200 nmol/L, respectively using the FCNTs modified GCE. The enhanced electrochemical response from the FCNTs modified GCE has been utilized in the evaluation of the chemical and biochemical behaviour of curcumin in presence of transition metal ions (Cu²⁺) and dsDNA, and the observation has been supported by the spectrochemical characteristics of the interactions.