Aim of the present work was to examine whether the SCOD (soluble chemical oxygen demand) released after the physical disruption of excess activated sludge can be used as an alternative carbon source for biological denitrification. In the first stage of research, we investigated the potential use of energy efficient hydrodynamic cavitation (HC) technique for the disruption of activated sludge. In a comparative study between ultrasonic cavitation (UC) and HC, it was observed that UC needs five times more energy than that of HC to release the same amount of SCOD. In the second stage of the experimental study, SCOD was successfully used as an alternative carbon source (alternative to sodium acetate) for biological denitrification. The critical weight ratio (SCOD/NO₃-N) of seven ensured 100% removal of nitrate. Nitrate removal kinetics indicated that denitrification with SCOD as a carbon source gives higher specific denitrification rate (by » 200%) as compared to conventional carbon source (sodium acetate).