Dormant axillarybudsexcised from crowns of pineapple (Ananas comosusL.,Merr.)culturedon growth regulator free Nitsch medium sprouted after 8-10 .d.Sprouted buds produced multiple shoots (7-10 shoots per bud) upon transfer to solidified Murashige and Skoog medium supplemented with 9.67μMNAA,9.84μMIBAand 9.29μMKIN. Each isolated shoot upon subculture to liquid medium of the same composition further proliferated to form more multiple shoots (60-65 shoots) and were maintained on a gyratory shaker (90-100 rpm).In vitro grown shoots were rooted on White medium supplemented with 0.54μMNAA and 1.97μMIBA.In vitro plantlets were established in cups with soilrite and hardened for four weeks.Phenotypic variants such as albinos,white streaked shoots and shoots with elongated internodes were observed in in vitro cultures.Approximately 520 in vitro produced plantlets were established in the field and these plants exhibit somaclonal variation. Thirty-eight plants were found to be yellowish,spineless with anthocyanin streaks and three wereanthocyanin rich, spined plants.