Extracellular polysaccharides (EPS) of a copper-sensitive (Cu^s) and a copper-resistant (Cu^r) Pseudomonas aeruginosa strain were investigated in terms of their production, chemical nature and response towards copper exposure. The extent of EPS synthesis by the resistant strain (4.78 mg mg⁻¹ cell dry wt.) was considerably higher over its sensitive counterpart (2.78 mg mg⁻¹ dry wt.). FTIR-spectroscopy and gas chromatography revealed that both the polymers were acidic in nature, containing alginate as the major component along with various neutral- and amino-sugars. Acid content in the Cu^r EPS (480.54 mg g⁻¹) was greater than that in the Cu^s EPS (442.0 mg g⁻¹). Presence of Cu²⁺ in the growth medium caused a dramatic stimulation (approximately 4-fold) in EPS synthesis by the Cu^r strain, while in a similar condition, the Cu^s failed to exhibit such response. The polymer of the resistant strain showed elevated Cu²⁺ binding (320 mg g⁻¹ EPS) compared to that of the sensitive type (270 mg g⁻¹). The overall observations show the potential of the Cu^r EPS for its deployment in metal bioremediation.