The presence of multiple sets of ParA and ParB genome partitioning proteins in bacteria harboring multi partite genomes raises doubt about their functional redundancy. Deinococcus radiodurans is a highly stress tolerant bacterium that harbors multipartite genome system comprising chromosome I, chromosome II and plasmids. Par As encoded on these elements were found t o be different in terms of their primary sequences and eventually clustered in the separate phylogenetic groups. As known earlier, ParB1 (ParB encoded on chromosome I) showed sequence specific interaction with its cognate cis element (centromere). ParA of chromosome I (ParA1) produced larger size complex in the presence of dsDNA and ATP, which was reduced to smaller size upon incubation with ParB1- centromere complex in vitro. GFP-ParA1 expressing in recombinant Escherichia coli showed a dynamic change in its cellular localization during cell division only when both ParB1 and centromere co-existed with ParA1. These results suggested that ParA1 requires ParB1 and its cognate centromere f or its cellular dynamics, which would eventually drive the separation of duplicated daughter chromosomes to opposite  poles during cell division.