An attempt was made to understand the sorption behaviour of Pu4+ and PuO22+ on Rhizopus arrhizus fungus. The sorption data were analyzed using Langmuir, Dubinin–Radushkevich, Freundlich isotherm and Tempkin isotherms, which revealed that the sorption proceeds via chemisorption through mono layer following Langmuir isotherm. the sorption kinetics were analyzed by different models revealing the predominance of pseudo 2nd order kinetics. Oxalic acid and sodium carbonate were used for effective stripping of Pu4+ and PuO22+, respectively. This biomass was found to be radiolytically stable and finally was applied for processing of SHLW from RR and FBR origins.