Uranium occurs naturally in groundwater and surface water. The objective of this study is to understand the causes for the occurrence of uranium and its spatio-temporal variation in groundwater in a part of Nalgonda district, Andhra Pradesh, south India. Uranium deposits occur in the southeastern part of this area. Groundwater samples were collected from 44 wells every two months from March 2008 to January 2009. The samples were analyzed for pH, ORP and uranium concentration. The uranium concentration in groundwater varies from 0.2 ppb to a maximum of 68 ppb with a mean of 18.5 ppb. About 21.6% of the samples were above the drinking water limit of 30 ppb set by USEPA. The uranium concentration varied with fluctuation in groundwater level, pH and ORP. Uranium concentration in groundwater changes depending on lithology, degree of weathering and rainfall recharge.