⁶⁴Cu is an useful radionuclide for both PET  imaging and targeted therapy, as it decays by three different modes, namely, electron capture (41%), β^-(40%) and positron emission (19%). ⁶⁴Cu is generally produced by ⁶⁴Ni (p, n) reaction in a cyclotron for medical use. High specific activity ‘no carrier added’ grade ⁶⁴Cu by ⁶⁴Zn (n, p) route is an alternative for research studies and was hence explored. 10 mg zinc foil target (48.63% in ⁶⁴Zn) was irradiated in the medium flux reactor Dhruva at a thermal neutron flux of ~5.6 x 10¹³ n cm^-2 s^-1 for 3 days. The irradiated Zn foil was dissolved in 5 mL 10 M HCl and ⁶⁴Cu was separated by anion exchange chromatography (Dowex 1 x 8; 100–200 mesh) at 3 M HCl conditions. ⁶⁴Cu radioactivity content and its radionuclide purity were ascertained by c-ray spectrometry using HPGe detector coupled to a 4 K multichannel analyser system. Radiochemical separation yielded a radionuclidic purity of 99.9% ⁶⁴Cu.