Bioremediation is an attractive option for the treatment of radioactive waste. We provide a proof of prin-ciple for augmentation of uranium bioprecipitation using the radiation inducible promoter, Pssb from Deinococcus radiodurans. Recombinant cells of D. radiodurans carrying acid phosphatase gene, phoN underthe regulation of Pssb when exposed to 7 kGy gamma radiation at two different dose rates of 56.8 Gy/minand 4 Gy/min, showed 8–9 fold increase in acid phosphatase activity. Highest whole cell PhoN activitywas obtained after 2 h in post irradiation recovery following 8 kGy of high dose rate radiation. Such cellsshowed faster removal of high concentrations of uranium than recombinant cells expressing PhoN undera radiation non-inducible deinococcal promoter, PgroESL and could precipitate uranium even after contin-uous exposure to 0.6 Gy/min gamma radiation for 10 days. Radiation induced recombinant D. radioduranscells when lyophilized retained high levels of PhoN activity and precipitated uranium efficiently. Theseresults highlight the importance of using a suitable promoter for removal of radionuclides from solution.