Solvent extraction studies on Sr²⁺ and Y³⁺ are carried out from varying concentrations (0.01-6.0M) of nitric acid using di-(2-ethylhexyl)phosphoric acid (D2EHPA) as extractant. Extraction of yttrium is observed to be higher than that of strontium at all the acidities and is found to increase substantially with decreasing concentration of nitric acid. Practically negligible extraction (D<10^-3) of Sr²⁺ is observed from feed solutions containing nitric acid in the range of 1.0 to 4.0M. These solvent extraction data are used to optimize the transport of ⁹⁰Sr and ⁹⁰Y across the supported liquid membrane (SLM) individually as well as from their mixture (due to insitue growth) under different experimental conditions. Selective separation of ⁹⁰Y (>90%) from ⁹⁰Sr is obtained in 6h, when the concentration of nitric acid in feed is kept at 1.0M and that of receiving phase is maintained at 4.0M. 20% D2EHPA in n-dodecane is found to be the optimum carrier concentration for the efficient transport of ⁹⁰Y in SLM mode. Under these conditions transport of strontium is found to be negligible. Radiochemical purity of the product ⁹⁰Y is checked by following its decay as well as by extraction paper chromatography. The contamination of ⁹⁰Sr in ⁹⁰Y product is found to be<0.001%. Based on the experimental results, a single stage SLM system for the generation of carrier-free ⁹⁰Y from ⁹⁰Sr source is described. The system is amenable for automation and scale up.