Yellow mosaic virus (YMV) disease is a serious disease which affects the black gram productivity. In this study, 10 molecular markers reported to be linked to YMV resistance in black gram and mungbean were validated on 19 diverse black gram genotypes for their utility in marker assisted selection. Three molecular markers (ISSR8111357, YMV1-FR and CEDG180) differentiated the YMV resistant and susceptible black gram genotypes. Other seven molecular markers were either monomorphic or failed to amplify the marker fragment in black gram genotypes. Inter Simple Sequence Repeat (ISSR) marker ISSR8111357 and its derived Sequence Characterized Amplified Region (SCAR) marker YMV1 –FR amplified the respective marker fragments in all YMV resistant genotypes except DPU 88-31, IPU 02-43, IPU 94-1 and IPU 07-3. A 136-bp allele of Simple Sequence Repeat (SSR) marker CEDG180 linked to YMV resistance was amplified in the aforementioned four genotypes in addition to PU 31. ISSR8111357 and CEDG180 are located on different linkage groups in the black gram genetic linkage map, suggesting that two independent resistance genes may be governing resistance to YMV in Indian black gram cultivars with marker ISSR8111357 linked to one resistance gene and marker CEDG180 linked to other resistance gene. Therefore, both markers can be used for marker assisted selection of YMV resistance. Among the resistant genotypes, PU 31 was the only genotype where both markers were amplified suggesting that PU 31 might be carrying both YMV resistance genes. Therefore, genotype PU 31 would be a valuable donor of YMV resistance and should be actively used in black gram breeding programs for incorporating YMV resistance.