This paper describes a facile and effective method to prepare ¹³³Ba source exploiting the intrinsic  properties of the anodized titanium to electro-deposit ¹³³Ba from an aqueous solution. The process consists of anodization of Ti rod [4.8mm (∅) × 10 mm (l)] to create a reactive surface to permit cathodic electrodeposition of ¹³³Ba from an aqueous solution. The factors that influence the electrodeposition of ¹³³Ba onto the anodized titanium substrate were identified and a careful control has been exercised to ensure optimum deposition of ¹³³Ba activity onto the substrate. The morphology of the electrodeposited samples were examined by SEM and EDS analyses. Sources containing ˜3.7–11.1MBq (0.1–0.3mCi) ¹³³Ba were prepared, encapsulated successfully in titanium capsules and subjected to quality control tests to ensure compliance with mandatory regulatory safety requirements.