The present study is aimed at carrying out a comparative performance evaluation of different types of ⁶⁸Ge/⁶⁸Ga generators to identify the best choice for use in ⁶⁸Ga-radiopharmacy. Over the 1 year period of evaluation, the elution yields from the CeO₂-based and SiO₂-based ⁶⁸Ge/⁶⁸Ga generators remained almost consistent, in contrast to the sharp decrease observed in the elution yields from TiO₂ and SnO₂-based generators. The level of ⁶⁸Ge impurity in ⁶⁸Ga eluates from the CeO₂ and SiO₂-based ⁶⁸Ge/⁶⁸Ga generator was always <10^-3%, while this level increased from 10^-3% to 10^-1% in case of TiO₂ and SnO₂-based generators. The level of chemical impurities in ⁶⁸Ga eluates from CeO₂ and SiO₂-based ⁶⁸Ge/⁶⁸Ga generators was negligibly low (<0.1 ppm) in contrast to the significantly higher level (1–20 ppm) of such impurities in eluates from other two generators. As demonstrated by radiolabeling studies carried out using DOTA-coupled dimeric cyclic RGD peptide derivative (DOTA-RGD₂), CeO₂-PAN and SiO₂-based generators are directly amenable for radiopharmaceutical preparation, whereas the other generators can be only used after post-elution purification of ⁶⁸Ga eluates. Clinically relevant dose of ⁶⁸Ga-DOTA-RGD₂ was prepared in a hospital radiopharmacy for non-invasive visualization of tumors in breast cancer patients using positron emission tomography.