Radiation induced graft copolymerization is pure, sterile and additive free method for the syn-thesis of hydrogels for biomedical applications. In the present work, attempt has been made toprepare the biocompatible, mucoadhesive hydrogels based on natural polysaccharide sterculia gum andpolyvinylpyrrolidone (PVP) for use as drug delivery devices. The effect of gamma radiation on swelling andvarious network parameters of hydrogels such as the polymer volume fraction in the swollen state (Φ),molecular weight of the polymer chain between two neighboring cross links (Mc), crosslink density (ρ),and mesh size (ξ) have been studied. Hydrogels have been characterized with scanning electron micro-graphs (SEMs), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction study (XRD), thermogravimetric analysis (TGA) and swelling studies. Swelling and mesh size decreased while gel strengthand crosslink density increased with increase in radiation dose. The swelling of hydrogels and releaseof drug ciprofloxacin from drug loaded hydrogels occurred through non-Fickian diffusion mechanism.These hydrogels have been observed to have non-thrombogenic, haemo-compatible and mucoadhesivenature and could be used as mucoadhesive drug delivery system to deliver drug to gastro intestinal tract(GIT) in controlled manner.