BARC/PUB/2004/0254

 
 

Cloning and Characterization of the DMC1 Genes in Oryza Sativa

 
     
 
Author(s)

Metkar, S. S.; Sainis, J. K.; Mahajan, S. K.
(MBD)

Source

Current Science, 2004. Vol. 87 (3): pp. 353-357

ABSTRACT

The DMC1 gene is a major homologous recombination gene, expressed during prophase I of meiosis. We have isolated and analysed two DMC1genes, viz. type A and type B from rice. It was observed that DMC1 type A is located on chromosome 12 whereas DMC1 type B is on chromosome 11. The location of DMC1 type A in the region on chromosome 12 that is duplicated on rice chromosome 11 is a new finding in this report. Earlier DMC1 orthologues have been reported on chromosomes 9 and 11. Partially overlapping 5 ′ and 3 ′ cDNAs of one of the DMC1 genes were obtained and used to generate the full - length DMC1 gene, which was cloned and over - expressed in E. coli.  

 
 
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