A total of 254 random, healthy individuals belonging to five distinct ethnic groups were analyzed for tetranucleotide repeat polymorphism at autosomal microsatellite locus D8S315. The five distinct ethnic groups: Ezhavas, Nairs, Arayas, Vishwakarma, and Muslims belonged to Kerala state of South India. Muslims are religio-ethnic group while other populations mentioned above belong to distinct section of Hindu religion. All these population groups speak “Malayalam,” an Indo-Dravidian language.