The effect of Zn and Cr doping on the transport and magnetic properties of MnV₂O₄ have been investigated  using resistivity, thermoelectric power (TEP), magnetization, neutron diffraction and X-ray diffraction techniques. It is observed, that with increase in Zn substitution the noncollinear orientation of Mn spins with the V spins decreases which effectively leads to the decrease of structural transition temperature more rapidly than Curie temperature. Investigations also show that with Zn doping both the Curie temperature (T_C) and structural transition temperature (T_S) decrease, while the gap between them increases rapidly. On the other hand, with Cr doping on the V site the T_C remains almost constant but T_S decreases rapidly. Moreover, with Zn doping both resistivity and TEP decrease, whereas with 10 % Cr doping the TEP decreases and a change of sign occurs indicating an increase in the band gap. This leads to the decrease of the mobility of the polaronic holes than the mobility of the electronic polarons at low temperature.