Being the world’s most severe parasitic infection, malaria causes more than a million deaths annually. Artemisin in and its derivatives are effective against malaria without any reputed cases of resistance. Thedrug artemisinin can be extracted from the leafy tissues of Artemisia annua L. Application of ionizingradiation to degrade natural bioactive agents, such as sodium alginate and then using them as growthpromoting substances is a novel emerging technology to exploit full genetic potential of crops in termsof growth, yield, and quality. In the present study, sodium alginate, irradiated by Co-60 gamma raystogether with various nitrogen doses, was used to study their effect on growth attributes, physiologi-cal and biochemical processes and production of artemisinin in A. annua. Of the various applied dosesof nitrogen, N₈₀(80 kg N ha⁻¹) together with ISA₈₀ (80 mg L⁻¹) proved superior in positively regulatingalmost all the parameters studied. Most importantly, an increase of 38.1 and 80.5% in artemisinin contentand yield respectively, was noticed at this treatment.