Polymer inclusion membranes (PIM) containing N,N,N´,N´ -tetra(2-ethylhexyl) diglycolamide(T2EHDGA) were evaluated for the separation of actinide ions such as Am³⁺, Pu⁴⁺, UO₂²⁺ and Th⁴⁺ from acidicfeeds. The PIMs were prepared using cellulose triacetate (CTA) as the polymer matrix, 2-nitrophenyloctyl ether (NPOE) as the plasticizer and T2EHDGA as the carrier extractant and the optimized membrane composition was found to be 68.4% T2EHDGA, 17.9% NPOE and 13.7% CTA which resulted in 74% Am³⁺ uptake at 1MHNO₃ in 2h. The uptake studies were carried out using feed solutions containing varying concentrations of nitric acid (0.5–3.0M) and showed the trend: Pu⁴⁺>Am³⁺>Th⁴⁺>UO₂²⁺.Quantitative stripping (>99%) of the sorbed Am³⁺ was possible using a solution containing 0.01M EDTA at pH 3.0. Reusability studies indicated deterioration of the PIM on continuous use.