Bakuchiol, extracted from the plant Psoralea corylifolia, has been proven to have antitumor, cytotoxic, antimicrobial and anti-inflammatory activity. In order to study if radiolabeled bakuchiol exhibits enhanced cytotoxicity, bakuchiol was radiolabeled with ¹²⁵I. In-vitro uptake studies of ¹²⁵I-bakuchiol were carried out using LS-A (lymphosarcoma) and barcl-95 (radiation-induced thymic lymphoma) ascitic and solid tumor cells of murine origin. In bot hLS-A and barcl-95, ¹²⁵I-bakuchiol showed significant uptake. Viability studies showed that the radioiodinated compound showed greater cytotoxic effect than bakuchiol.