The accuracy of radioactive particle tracking technique depends on the ability to locate the exact position of a tracer particle. In the current work, experiments are performed to find the accuracy of an RPT measurement in terms of locating the position of the tracer particle and its velocity measurement. Results are presented for different tracer velocities at different data acquisition frequencies to minimize the error generated due to dynamic bias. Finally an optimal frequency is recommended for different velocities of a tracer particle to achieve the best possible accuracy.