We report levels of polycyclic aromatic hydrocarbons in sediment, seawater, fish and crab samples from Thane creek, India. Concentrations of sixteen USEPA PAH in sediments varies from 874 to 1925 ng g−1, dry weight (dw) in winter, between 219–495 ng g−1 in summer. In seawater Σ16 PAHs concentration during winter was recorded 706 ± 193 ng L−1, and during summer 337 ± 79 ng L−1. Total concentrations of PAHs in consumable portion of lizard fish, bombay duck and crab were found 156.8 ± 18 and 122 ± 24.5 ng g−1, 117.4 ± 17.65 ng g−1and 95.8 ± 16.2 ng g−1, 348 ± 94.5 and 95.62 ± 31.9 ng g−1 wet weight (ww) in summer and winter respectively. PAHs concentration were compared with sediments quality guidelines viz. ERL-ERM, TEL-PEL indexes for finding ecotoxicological risk on marine organism. The ILCR values werea bove public screening criteria for carcinogens.