Cyanex-923 has been used as the stationary phase in the extraction chromatographic separation of actinide ions from simulated high-level waste (SHLW). Chromosorb-W was used as the solid inert support. The uptake behaviour of U(VI), Pu(IV), and Am(III) was studied by batch studies. Breakthrough capacity of the column for Am(III) in the presence of macro amount of Eu(III) has been determined in three successive cycles of loading and elution. The influence of interfering ions like Fe(III), Cr(VI), Al(III), and Ni(II), etc. has been investigated. Effect of column parameters like diameter and height on Am(III) elution profile has also been studied.