A study has been done to assess the uranium intake through drinking water. The area of study is twin cities of Hyderabad and Secunderabad, India. Uranium concentration in water samples was analysed by laser-induced fluorimetry. The associated agedependent uranium intake was estimated by taking the prescribed water intake values. The concentration of uranium varies from below detectable level (minimum detectable level0.20±0.02 µg l^-1) to 2.50±0.18 µg l^-1, with the geometric mean (GM) of 0.67 µg l^-1 in tap water, whereas in ground water, the range is 0.60±0.05 to 82±7.1 µg l^-1 with GM of 10.07 µg l^-1. The daily intake of uranium by drinking water pathway through tap water for various age groups is found to vary from 0.14 to 9.50 µg d^-1 with mean of 1.55 µg d^-1.