The efficiency of tailor made immobilized Saccharomyces cerevisiae (biomatrix) for the sorption of radionuclides ²³³U, ²³⁹Pu, ²⁴¹Am, ¹³⁷Ce,¹⁴⁴C_s, ¹⁰³,¹⁰⁶ Ru and ⁹⁰ Sr from aqueous nitrate solutions at different pH was studied. Effect of ionic strength, anionic components, initial metal concentration and particle size of the biomatrix on the sorption of metal ions were investigated. At pH in the range of 1 to 2 more than 95% sorption of U, Pu, Am and Ce could be accomplished, while that of Ru was 65%. Sorption of Cs and Sr were negligible under simil ar conditions.The metal ion-biomatrix system for Pu, Am and Ce reached equilibrium within 60 minutes. In the case of U, equilibrium attained in 100 minutes.The presence of anionic components, Cl–, C₂O₄^2–, CH₃COO^–, NO₃^–and SO₄^2–(up to 0.5 mol.dm^–3 of their individual concentration) in the aqueous solutions has no effect on the sorption of Pu by the biomatrix. Sorption of U, Pu, Am were observed in the presence of several cationic impurities such as Al, Be, Cd, Cr, Fe, Mn, Pb, Ce, Dy, Eu, Gd and Sm. Metal sorbed on the biomatrix could be leached out using 5 mol.dm^–3nitric acid. The I.R spectra of U bearing biomatrix suggest chemical interaction of uranyl ion with the biomatrix.