This study is an extension of a previous study undertaken to rejuvenate ammonia cracker tubes of Alloy 625 alloy that have been service exposed in heavy water plants for their full service life of 100,000 h. The service exposure caused significant microstructural modifications and deterioration in mechanical properties, and a solution annealing treatment of 2 h at 1160°C rejuvenated all properties similar to those of the virgin alloy. The present study reports the evolution of microstructure and mechanical properties of a full service exposed centrifugally cast Alloy 625 tube that was put into service again for 55,000 h after receiving a rejuvenation treatment. During the second service, microstructural modifications, increase in strength and loss of ductility were on the lines of the work reported earlier. However, it was encouraging to observe that degraded properties after the second service life remained within the bounds of those of virgin and full service exposed tubes. The good performance of the re juvenated tube during the second service life has been attributed to good control of operation parameters that limited the precipitation of grain boundary carbides during the first service life, which otherwise would have had a direct bearing on premature failure of tubes during their second service life.