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Author(s) |
Chanda, A.; Rawat, K. P.; Khader, S. A.; Padmanabhan, D.; Sarma, K. S. S.; Samuel, G. (RTDD)
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Source |
Journal of Radioanalytical & Nuclear Chemistry, 2014. Vol. 302 (2): pp. 989-992 |
ABSTRACT
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In-house
sterilization by electron beam (EB) radiation of various plastic
consumables (plastic petridishes, micro centrifugal tubes and
screw-capped vials) used routinely in the lab was studied by use of
three microbiological cultures (S. aureus, Bacillus subtilis and Candida
albicans). Current international standards (ISO 11137-Part 2 -ISO,
2011) recommend an irradiation dose of 25 kGy as a reference dose for
terminal sterilization. All containers were exposed in an ILU-6, 2 MeV,
20 kW, Pulse EB accelerator located in our complex. Sterility test
(S.T.) was performed and results revealed that 106 and 107 population of all strains passed whereas 108
population failed S.T. for all micro-organisms indicating the potential
of 2 MeV EB for commercial sterilization of plastic lab consumables for
up to 107 population of these microorganisms.` |
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