The glucose concentration in human blood is an essential predictor for diagnosing and monitoring symptoms related to diabetes mellitus. In the current scenario of increasing diabetes patients daily, developing costeffective glucose (GLU) sensors with excellent selectivity and sensitivity is relevant. The present study investigates the potential application of pristine and transition metal (TM = Sc, Pd, and Au) functionalized 2D transition metal dichalcogenide (TMDC) MoTe₂ for glucose detection using first-principles Density Functional theory. The results demonstrate that the Pd and Sc functionalized systems are more highly sensitive towards the GLU than the pristine MoTe₂, where the GLU adsorption is weak with an adsorption energy of -0.424 eV. The satisfactory adsorption energy (-0.8 eV), accelerated charge transfer and short recovery time (28 s) at 300 K revealed that Pd-functionalized MoTe₂ could be a promising candidate for room-temperature GLU detection. The interaction of Pd on MoTe₂ is due to charge transfer from Pd to MoTe₂, whereas the adsorption of glucose on the Pd + MoTe₂ system is facilitated through the charge  transfer from the O 2p orbital of GLU to Pd. The firstprinciple Molecular dynamic simulations at 500 K validated the high-temperature structural stability of the MoTe₂ + Pd-based GLU sensor. This research will give a solid theoretical foundation for the experimental fabrication of MoTe₂-based glucometers.